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Journal of Clinical Microbiology, November 2005, p. 5477-5482, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5477-5482.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Mutations Associated with Isoniazid Resistance in Mycobacterium tuberculosis Isolates from China

Min Zhang,1 Jun Yue,2 Yan-ping Yang,1 Hong-mei Zhang,1 Jian-qiang Lei,1 Rui-liang Jin,1 Xue-lian Zhang,1 and Hong-hai Wang1*

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University,1 Shanghai Pneumology Hospital, Shanghai 200433, People's Republic of China2

Received 11 May 2005/ Returned for modification 16 June 2005/ Accepted 15 August 2005

Nine structural genes (furA, katG, inhA, kasA, Rv0340, iniB, iniA, iniC, and efpA) and two regulatory regions (the oxyR-ahpC intergenic region and the promoter of mabA-inhA) in 87 isoniazid (INH)-monoresistant and 50 INH-susceptible Mycobacterium tuberculosis isolates collected from five provinces of China were analyzed by sequencing. Eighty-two (94.3%) INH-resistant isolates had mutations in the katG gene, with the katG Ser315Thr mutation predominant (55.2%). No mutation at codon 463 of katG was detected among the 50 INH-susceptible isolates with different IS6110 fingerprints. In addition, there were 35 (40.2%) INH-resistant isolates that had a mutation at codon 463 of katG. Of the INH-resistant strains, 20 (23.0%) isolates harbored double mutations at two separate loci of katG. Mutations in the inhA promoter region occurred in 13 (14.9%) isolates; 4.6% of the isolates had inhA structural gene mutations, and 11.5% harbored mutations in the oxyR-ahpC intergenic region. Drug resistance-associated mutations were detected in the iniBAC region and efpA.

* Corresponding author. Mailing address: Institute of Genetics, Fudan University, 220 Handan Rd., Shanghai 200433, People's Republic of China. Phone: 86 21 65643777. Fax: 86 21 65648376. E-mail: hhwang{at}fudan.edu.cn.

Journal of Clinical Microbiology, November 2005, p. 5477-5482, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5477-5482.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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