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Journal of Clinical Microbiology, November 2005, p. 5639-5641, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5639-5641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Techniques for Detection of Carbapenem-Resistant Klebsiella pneumoniae in Stool Surveillance Cultures

D. Landman,* J. K. Salvani, S. Bratu, and J. Quale

Department of Medicine, SUNY-Downstate Medical Center, Brooklyn, New York

Received 5 July 2005/ Returned for modification 15 August 2005/ Accepted 2 September 2005

Screening for gastrointestinal colonization with multidrug-resistant nosocomial pathogens is an important component of infection control protocols. In the New York City region, carbapenem-resistant Klebsiella pneumoniae strains, which harbor the KPC carbapenem-hydrolyzing ß-lactamase, have rapidly emerged. The potential utility of screening medium, which involved using 10-µg imipenem disks, was investigated. The method of placing a sample from a fecal surveillance culture into broth containing an imipenem disk appeared to have the greatest sensitivity for detecting KPC-producing K. pneumoniae. Gastrointestinal colonization with two other carbapenem-resistant nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii, was also detected using this method. Placing fecal surveillance specimens into broth containing an imipenem disk is an easy method for screening samples for carbapenem-resistant nosocomial pathogens.

* Corresponding author. Mailing address: Division of Infectious Diseases, Box 77, SUNY Downstate, 450 Clarkson Ave., Brooklyn, NY 11203. Phone: (718) 270-3790. Fax: (718) 270-2468. E-mail: dlandman{at}downstate.edu.

Journal of Clinical Microbiology, November 2005, p. 5639-5641, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5639-5641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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