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Journal of Clinical Microbiology, November 2005, p. 5768-5770, Vol. 43, No. 11
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.11.5768-5770.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Supplementation of CHROMagar Candida Medium with Pal's Medium for Rapid Identification of Candida dubliniensis
Ismail H. Sahand,1
María D. Moragues,2
Elena Eraso,1
María Villar-Vidal,1
Guillermo Quindós,1 and
José Pontón1*
Departamento de Inmunología, Microbiología, y Parasitología, Facultad de Medicina y Odontología,1
Departamento de Enfermería I, Universidad del País Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain2
Received 21 April 2005/
Returned for modification 4 July 2005/
Accepted 15 August 2005
CHROMagar Candida medium is used for the isolation and identification of Candida species, but it does not differentiate Candida albicans from Candida dubliniensis. This differentiation can be achieved by using Pal's agar, which cannot be used in primary isolation. We have combined both media to obtain a new medium that can be used for the isolation and identification of C. dubliniensis in primary cultures.
* Corresponding author. Mailing address: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain. Phone: 34 94-601-2855. Fax: 34 94-601-3400. E-mail:
jose.ponton{at}ehu.es.
Journal of Clinical Microbiology, November 2005, p. 5768-5770, Vol. 43, No. 11
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.11.5768-5770.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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