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Use of a Real-Time PCR TaqMan Assay for Rapid Identification and Differentiation of Burkholderia pseudomallei and Burkholderia mallei

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640,¹ Los Alamos National Laboratory, Los Alamos, New Mexico 87545²

Received 8 June 2005/ Returned for modification 19 July 2005/ Accepted 24 August 2005

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

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