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Journal of Clinical Microbiology, November 2005, p. 5794-5795, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5794-5795.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Increased Rate of DNA Recovery from United Kingdom Epidemic Clostridium difficile PCR Ribotype 1 Strains Stored Cryogenically

N. J. Asha, W. N. Fawley, J. Freeman, and M. H. Wilcox*

Department of Microbiology, Leeds Teaching Hospitals, Leeds LS1 3EX, United Kingdom

Received 13 May 2005/ Returned for modification 6 July 2005/ Accepted 31 August 2005

We noted that some Clostridium difficile isolates are nonrecoverable after frozen storage and so used molecular typing analysis to characterize DNA from these strains. The recovery rate of C. difficile PCR ribotype 1 was statistically significantly greater than that of other strains. This observation has implications for C. difficile epidemiological studies.

* Corresponding author. Mailing address: Department of Microbiology, Leeds General Infirmary, The Old Medical School, Thoresby Place, Leeds LS1 3EX, United Kingdom. Phone: 44 113 392 6818. Fax: 44 113 343 5649. E-mail: Mark.Wilcox{at}leedsth.nhs.uk.

Journal of Clinical Microbiology, November 2005, p. 5794-5795, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5794-5795.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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