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CASE REPORT

Non-Culture-Based Analysis of Bacterial Populations from Patients with Chronic Rhinosinusitis

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand,¹ BLIS Technologies Limited, Center for Innovation, University of Otago, Dunedin, New Zealand,² Otolaryngology and Head and Neck Surgery, Department of Medical and Surgical Sciences, University of Otago, Dunedin, New Zealand³

Received 13 June 2005/ Returned for modification 20 July 2005/ Accepted 9 August 2005

Middle meatus aspirates from patients with chronic rhinosinusitis were analyzed by bacterial culture, denaturing gradient gel electrophoresis (DGGE), and antibiotic sensitivity techniques. DGGE detected a greater bacterial diversity than culture methods. Although resistance to antibiotics was low, there was evidence of changes in the composition of the bacterial microbiota over time, and the presence of noncultured bacteria was demonstrated.