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Journal of Clinical Microbiology, December 2005, p. 5912-5915, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.5912-5915.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Denaturing High-Performance Liquid Chromatography for Rapid Detection and Identification of Seven Candida Species

Oliver Goldenberg,1,{dagger} Stefanie Herrmann,1,{dagger} Thomas Adam,1 Gina Marjoram,1 George Hong,2 Ulf B. Göbel,1 and Barbara Graf1*

Institute of Microbiology and Hygiene, Charité Universitaetsmedizin Berlin, Campus Charité Mitte, Dorotheenstraße 96, D-10117 Berlin, Germany,1 Transgenomic Ltd., Omaha, Nebraska2

Received 11 April 2005/ Returned for modification 5 June 2005/ Accepted 22 September 2005

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

* Corresponding author. Mailing address: Institute of Microbiology and Hygiene, Charité Universitaetsmedizin Berlin, Campus Charité Mitte, Dorotheenstraße 96, D-10117 Berlin, Germany. Phone: 49-30-450-524229. Fax: 49-30-450-524908. E-mail: barbara.graf{at}charite.de.

{dagger} O.G. and S.H. contributed equally to this work.

Journal of Clinical Microbiology, December 2005, p. 5912-5915, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.5912-5915.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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