This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reinert, R. R.
Right arrow Articles by Al-Lahham, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reinert, R. R.
Right arrow Articles by Al-Lahham, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2005, p. 6035-6041, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6035-6041.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Relationship between the Original Multiply Resistant South African Isolates of Streptococcus pneumoniae from 1977 to 1978 and Contemporary International Resistant Clones

Ralf René Reinert,1* Michael R. Jacobs,2 Peter C. Appelbaum,3 Saralee Bajaksouzian,2 Soraia Cordeiro,2 Mark van der Linden,1 and Adnan Al-Lahham1

Institute of Medical Microbiology, National Reference Centre for Streptococci, Aachen, Germany,1 University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 44106,2 Hershey Medical Center, Hershey, Pennsylvania 170333

Received 13 June 2005/ Returned for modification 9 August 2005/ Accepted 19 September 2005

High-level penicillin G-resistant as well as multidrug-resistant Streptococcus pneumoniae isolates were first described in South Africa in 1977. The relationship between these original multidrug-resistant South African isolates and other resistant clones was investigated. Twenty-six representative isolates isolated from initial outbreaks in South Africa from 1977 to 1978 were characterized by multilocus sequence typing and pulsed-field gel electrophoresis. Twenty-one isolates were penicillin resistant and five were penicillin intermediate, with variable susceptibilities to macrolides, clindamycin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole. Fourteen isolates were serotype 19A, 11 were serotype 6A, and one was serotype 14. Penicillin-resistant serotype 19A isolates belonged to three closely related sequence types (STs), ST 41 (n = 6), ST 1605 (n = 3), and ST 1656 (n = 1). Penicillin-resistant serotype 6A isolates belonged to two closely related STs, ST 1094 (n = 10) and ST 1607 (n = 1), and were not closely related to other international clones. The serotype 14 penicillin-intermediate isolate was not closely related to the other isolates from South Africa but was a predicted founder of a clonal group with 41 different STs. Five new STs, ST 1605, ST 1607, ST 1608, ST 1610, and ST 1656, are described for the first time in this study. New molecular methods have characterized the original multiply resistant South African pneumococcal isolates from 1977 to 1978 and have shown the relationships of these clones to major pneumococcal clones.

* Corresponding author. Mailing address: Institute for Medical Microbiology, National Reference Center for Streptococci, University Hospital, RWTH, Pauwelsstr. 30, Aachen, Germany. Phone: 49 241 8089787. Fax: 49 241 8082483. E-mail: Reinert{at}rwth-aachen.de.

Journal of Clinical Microbiology, December 2005, p. 6035-6041, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6035-6041.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacobs, M. R., Good, C. E., Beall, B., Bajaksouzian, S., Windau, A. R., Whitney, C. G. (2008). Changes in Serotypes and Antimicrobial Susceptibility of Invasive Streptococcus pneumoniae Strains in Cleveland: a Quarter Century of Experience. J. Clin. Microbiol. 46: 982-990 [Abstract] [Full Text]  
  • Al-Lahham, A., Appelbaum, P. C., van der Linden, M., Reinert, R. R. (2006). Telithromycin-Nonsusceptible Clinical Isolates of Streptococcus pneumoniae from Europe. Antimicrob. Agents Chemother. 50: 3897-3900 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»