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Journal of Clinical Microbiology, December 2005, p. 6042-6047, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6042-6047.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR-Amplified Fragment Length Polymorphism Analysis of Methicillin-Resistant Staphylococcus aureus

Anneke van der Zee,1* Max Heck,2 Marcel Sterks,1 Airien Harpal,1 Emile Spalburg,2 Leonard Kazobagora,1 and Wim Wannet2

Laboratory of Medical Microbiology, St. Elisabeth Hospital, Tilburg, The Netherlands,1 Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands2

Received 29 July 2005/ Accepted 10 September 2005

Multienzyme multiplex PCR-amplified fragment length polymorphism (ME-AFLP) typing is a reliable and simple method for typing of bacterial species. In this study we analyzed two well-documented strain collections of Staphylococcus aureus and compared ME-AFLP typing results with results of various other typing methods. The discriminatory power of ME-AFLP was found comparable to pulsed-field gel electrophoresis, and typing results were highly concordant. ME-AFLP typing presents a suitable method for prescreening of large strain collections. Furthermore, the obtained typing patterns were found to cluster according to the staphylococcal cassette chromosome mec types of the strains.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology, St. Elisabeth Hospital, PO Box 747, 5000AS Tilburg, The Netherlands. Phone: 0031-13-5392653. Fax: 0031-13-5441264. E-mail: a.vd.zee{at}elisabeth.nl.

Journal of Clinical Microbiology, December 2005, p. 6042-6047, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6042-6047.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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