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Journal of Clinical Microbiology, December 2005, p. 6098-6107, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6098-6107.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Association of Virulence Genotype with Phylogenetic Background in Comparison to Different Seropathotypes of Shiga Toxin-Producing Escherichia coli Isolates

Jean Pierre Girardeau,^1* Alessandra Dalmasso,^1, Yolande Bertin,¹ Christian Ducrot,² Séverine Bord,² Valérie Livrelli,³ Christine Vernozy-Rozand,⁴ and Christine Martin¹

Unité de Microbiologie, Centre de Recherche INRA de Clermont-Ferrand-Theix, 63122 Saint-Genes Champanelle, France,¹ Groupe de Recherche Pathogénie Bactérienne Intestinale, Faculté de Pharmacie, Université d'Auvergne Clermont 1, 63001 Clermont-Ferrand Cedex, France,³ Unité d'Epidémiologie Animale, INRA, Centre de Recherche INRA de Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France,² Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France⁴

Received 26 May 2005/ Returned for modification 1 August 2005/ Accepted 7 September 2005

The distribution of virulent factors (VFs) in 287 Shiga toxin-producing Escherichia coli (STEC) strains that were classified according to Karmali et al. into five seropathotypes (M. A. Karmali, M. Mascarenhas, S. Shen, K. Ziebell, S. Johnson, R. Reid-Smith, J. Isaac-Renton, C. Clark, K. Rahn, and J. B. Kaper, J. Clin. Microbiol. 41:4930-4940, 2003) was investigated. The associations of VFs with phylogenetic background were assessed among the strains in comparison with the different seropathotypes. The phylogenetic analysis showed that STEC strains segregated mainly in phylogenetic group B1 (70%) and revealed the substantial prevalence (19%) of STEC belonging to phylogenetic group A (designated STEC-A). The presence of virulent clonal groups in seropathotypes that are associated with disease and their absence from seropathotypes that are not associated with disease support the concept of seropathotype classification. Although certain VFs (eae, stx_2-EDL933, stx_2-vha, and stx_2-vhb) were concentrated in seropathotypes associated with disease, others (astA, HPI, stx_1c, and stx_2-NV206) were concentrated in seropathotypes that are not associated with disease. Taken together with the observation that the STEC-A group was exclusively composed of strains lacking eae recovered from seropathotypes that are not associated with disease, the "atypical" virulence pattern suggests that STEC-A strains comprise a distinct category of STEC strains. A practical benefit of our phylogenetic analysis of STEC strains is that phylogenetic group A status appears to be highly predictive of "nonvirulent" seropathotypes.

Present address: Facoltà di Medicina Vétérinaria Università degli Studi di Torino, Turin, Italy.

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