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Journal of Clinical Microbiology, December 2005, p. 6113-6116, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6113-6116.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

DNA Polymorphism and Molecular Subtyping of the Capsular Gene Cluster of Group B Streptococcus{dagger}

Shannon D. Manning,1,2 David W. Lacher,1 H. Dele Davies,2 Betsy Foxman,3 and Thomas S. Whittam1*

National Food Safety and Toxicology Center,1 Department of Pediatrics and Human Development, Michigan State University,2 Department of Epidemiology and Center for Molecular and Clinical Epidemiology of Infectious Diseases, University of Michigan School of Public Health, East Lansing, Michigan 488243

Received 26 May 2005/ Returned for modification 27 June 2005/ Accepted 6 September 2005

Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

* Corresponding author. Mailing address: Microbial Evolution Laboratory, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824. Phone: (517) 432-3100, ext. 178. Fax: (517) 432-2310. E-mail: whittam{at}msu.edu.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, December 2005, p. 6113-6116, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6113-6116.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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