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Journal of Clinical Microbiology, December 2005, p. 6117-6119, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6117-6119.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Commercially Available Line Probe Assay for Genotyping of Hepatitis C Virus 5a Strains

Jannick Verbeeck,1 Piet Maes,1 Elke Wollants,1 Schalk Van der Merwe,2 Ernie Song,3 Frederik Nevens,4 and Marc Van Ranst1*

Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium,1 Hepatology/GI Research Laboratory, Department of Internal Medicine and Gastroenterology, University of Pretoria, Pretoria, South Africa,2 Department of Internal Medicine, University of Witwatersrand, Witwatersrand, South Africa,3 Division of Hepatology, University Hospital Gasthuisberg, Leuven, Belgium4

Received 16 May 2005/ Returned for modification 24 August 2005/ Accepted 16 September 2005

To validate a commercially available line probe assay for samples containing the infrequently found hepatitis C virus (HCV) genotype 5a, we sequenced a 511-nucleotide fragment of the NS4b region of Belgian and South African HCV genotype 5a samples. Phylogenetic analysis of the sequence data was performed. For the 77 HCV genotype 5a samples collected, there was 100% concordance between the genotype assignment by the line probe assay and the genotyping based on nucleotide sequencing, despite sequence heterogeneity in the probe binding sites of some samples.

* Corresponding author. Mailing address: Laboratory of Clinical and Epidemiological Virology, Minderbroedersstraat 10, B-3000 Leuven, Belgium. Phone: 32-16-347908. Fax: 32-16-347900. E-mail: marc.vanranst{at}uz.kuleuven.ac.be.

Journal of Clinical Microbiology, December 2005, p. 6117-6119, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6117-6119.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Copyright © 2005 by the American Society for Microbiology. All rights reserved.