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Journal of Clinical Microbiology, December 2005, p. 6139-6143, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6139-6143.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay That Identifies the Major Lipooligosaccharide Serotype Expressed by Moraxella catarrhalis Clinical Isolates

Katie J. Edwards,1,2 Johanna M. Schwingel,1,2 Anup K. Datta,4 and Anthony A. Campagnari1,2,3*

Department of Microbiology and Immunology,1 Witebsky Center for Microbial Pathogenesis and Immunology,2 Department of Medicine, State University of New York at Buffalo, Buffalo, New York 14214,3 Glycobiology Research and Training Center, Department of Molecular and Cellular Medicine, University of California, San Diego, La Jolla, California 92093-06874

Received 20 September 2005/ Accepted 27 September 2005

A heterologous cluster of glycosyltransferase genes was identified in the three Moraxella catarrhalis LOS serotype strains. Multiple PCR primers designed to this region amplified products that differentiate between the serotypes more rapidly and efficiently than previously described serological analyses. This assay will be valuable for clinical and research-based studies.

* Corresponding author. Mailing address: Department of Microbiology, University at Buffalo, Biomedical Research Bldg. Rm. 143, 3435 Main St., Buffalo, NY 14214. Phone: (716) 829-2673. Fax: (716) 829-3889. E-mail: aac{at}buffalo.edu.

Journal of Clinical Microbiology, December 2005, p. 6139-6143, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6139-6143.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schwingel, J. M., Edwards, K. J., Cox, A. D., Masoud, H., Richards, J. C., St. Michael, F., Tekwe, C. D., Sethi, S., Murphy, T. F., Campagnari, A. A. (2009). Use of Moraxella catarrhalis Lipooligosaccharide Mutants To Identify Specific Oligosaccharide Epitopes Recognized by Human Serum Antibodies. Infect. Immun. 77: 4548-4558 [Abstract] [Full Text]  
  • Schwingel, J. M, Michael, F. S., Cox, A. D, Masoud, H., Richards, J. C, Campagnari, A. A (2008). A unique glycosyltransferase involved in the initial assembly of Moraxella catarrhalis lipooligosaccharides. Glycobiology 18: 447-455 [Abstract] [Full Text]  
  • Verhaegh, S. J. C., Streefland, A., Dewnarain, J. K., Farrell, D. J., van Belkum, A., Hays, J. P. (2008). Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002. Microbiology 154: 1178-1184 [Abstract] [Full Text]  


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