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Journal of Clinical Microbiology, December 2005, p. 6147-6149, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6147-6149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Ryan R. McDonald,^1* Nick A. Antonishyn,¹ Toni Hansen,¹ Laelie A. Snook,^1, Evelyn Nagle,¹ Michael R. Mulvey,² Paul N. Levett,¹ and Greg B. Horsman¹

Provincial Laboratory, Saskatchewan Health, Regina, Saskatchewan, Canada,¹ National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada²

Received 21 September 2005/ Accepted 4 October 2005

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

Present address: Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.

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