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Journal of Clinical Microbiology, December 2005, p. 6152-6154, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6152-6154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection and Serotyping of Streptococcus pneumoniae from Nasopharyngeal Samples by PCR-Based Multiplex Assay

Jaime Moreno, Elkin Hernández, Olga Sanabria, and Elizabeth Castañeda*

Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia

Received 13 May 2005/ Returned for modification 3 July 2005/ Accepted 17 September 2005

We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.

* Corresponding author. Mailing address: Instituto Nacional de Salud, Avenida calle 26 No. 51-60, Bogotá, Colombia. Phone: 57-1-220-0920. Fax: 57-1-220-0901. E-mail: ecastaneda{at}ins.gov.co.

Journal of Clinical Microbiology, December 2005, p. 6152-6154, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6152-6154.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rubin, L. G., Rizvi, A., Baer, A. (2008). Effect of Swab Composition and Use of Swabs versus Swab-Containing Skim Milk-Tryptone-Glucose-Glycerol (STGG) on Culture- or PCR-Based Detection of Streptococcus pneumoniae in Simulated and Clinical Respiratory Specimens in STGG Transport Medium. J. Clin. Microbiol. 46: 2635-2640 [Abstract] [Full Text]  


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