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Journal of Clinical Microbiology, December 2005, p. 6176-6182, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6176-6182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Identification of Adenoviruses in Clinical Samples by Analyzing a Partial Hexon Genomic Region{dagger}

I. Casas,1 A. Avellon,1 M. Mosquera,2 O. Jabado,3 J. E. Echevarria,2 R. H. Campos,4 M. Rewers,5 P. Perez-Breña,1 W. I. Lipkin,3 and G. Palacios3*

Servicio de Virología,1 Servicio de Microbiología Diagnóstica, Centro Nacional de Microbiología, ISCIII, Madrid, Spain,2 The Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, New York, New York,3 Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina,4 and Barbara Davis Center for Diabetes Research, University of Colorado Health Sciences Center, Denver Colorado5

Received 1 June 2005/ Returned for modification 8 July 2005/ Accepted 7 September 2005

Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.

* Corresponding author. Mailing address: Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 772 W. 168th Street, Floor 18, New York, NY 10032. Phone: (212) 342-9034. Fax: (212) 342-9044. E-mail: gp2050{at}columbia.edu.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, December 2005, p. 6176-6182, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6176-6182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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