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Journal of Clinical Microbiology, December 2005, p. 6189-6193, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6189-6193.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Microelectronic Array Technology for Rapid Identification of Clinically Relevant Mycobacteria

Maurizio Sanguinetti,1*,§ Linda Novarese,1,§ Brunella Posteraro,1,§ Stefania Ranno,1 Elena De Carolis,1 Giovanni Pecorini,1 Barbara Lucignano,2 Fausta Ardito,1 and Giovanni Fadda1

Istituto di Microbiologia, Università Cattolica del Sacro Cuore,1 Laboratorio di Microbiologia, Ospedale Pediatrico "Bambino Gesù," Rome, Italy2

Received 30 May 2005/ Returned for modification 1 August 2005/ Accepted 6 September 2005

We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.

* Corresponding author. Mailing address: Istituto di Microbiologia, Largo F. Vito, 1, 00168 Rome, Italy. Phone: 39 630154964. Fax: 39 63051152. E-mail: msanguinetti{at}rm.unicatt.it.

§ These authors contributed equally to this work.

Journal of Clinical Microbiology, December 2005, p. 6189-6193, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6189-6193.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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