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Journal of Clinical Microbiology, December 2005, p. 6194-6196, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6194-6196.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

PCR Protocol for Specific Identification of Candida nivariensis, a Recently Described Pathogenic Yeast

Julia Alcoba-Flórez,1,2 María del Pilar Arévalo,5 F. Javier González-Paredes,2 Josep Cano,4 Josep Guarro,4 Eduardo Pérez-Roth,2 and Sebastián Méndez-Álvarez2,3*

Department of Microbiology, Hospital Universitario N. S. de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain,1 Research Institute, Hospital Universitario N. S. de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain,2 Department of Cellular Biology & Microbiology, University of La Laguna, Canary Islands, Spain,3 Unitat de Microbiologia, Facultat de Medicina i Cièncias de la Salut, Universitat Rovira i Virgili, Reus, Tarragona, Spain,4 Department of Public Health, School of Medicine, University of La Laguna, Tenerife, Spain5

Received 21 July 2005/ Returned for modification 20 September 2005/ Accepted 30 September 2005

Candida nivariensis is a recently described pathogenic yeast closely related to Candida glabrata. We developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rRNA gene for the rapid identification of C. nivariensis. PCR with these primers amplified a 206-bp amplicon from C. nivariensis.

* Corresponding author. Mailing address: Unidad de Investigación, H. U. Ntra. Sra. de Candelaria, Ctra. Del Rosario s/n, 38010 Santa Cruz de Tenerife, Spain. Phone: 34922600080. Fax: 34922600562. E-mail: smenalv{at}gobiernodecanarias.org.

Journal of Clinical Microbiology, December 2005, p. 6194-6196, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6194-6196.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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