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Journal of Clinical Microbiology, February 2005, p. 703-710, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.703-710.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sequence Analysis of the Escherichia coli O15 Antigen Gene Cluster and Development of a PCR Assay for Rapid Detection of Intestinal and Extraintestinal Pathogenic E. coli O15 Strains

Lothar Beutin,1 Jiang Tao,2 Lu Feng,2,3* Gladys Krause,1 Sonja Zimmermann,1 Kerstin Gleier,1 Qiuyu Xia,2 and Lei Wang2,3,4*

Division of Microbial Toxins, Robert Koch Institute, Berlin, Germany,1 TEDA School of Biological Sciences and Biotechnology,2 Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University,3 Tianjin Biochip Corp., Tianjin, People's Republic of China4

Received 20 June 2004/ Returned for modification 18 September 2004/ Accepted 29 September 2004

A collection of 33 Escherichia coli serogroup O15 strains was studied with regard to O:H serotypes and virulence markers and for detection of the O-antigen-specific genes wzx and wzy. The strains were from nine different countries, originated from healthy or diseased humans and animals and from food, and were isolated between 1941 and 2003. On the basis of virulence markers and clinical data the strains could be split into different pathogroups, such as uropathogenic E. coli, enteropathogenic E. coli, Shiga toxin-producing E. coli, and enteroaggregative E. coli. H serotyping and genotyping of the flagellin (fliC) gene revealed 11 different H types and a close association between certain H types, virulence markers, and pathogroups was found. Nucleotide sequence analysis of the O-antigen gene cluster revealed putative genes for biosynthesis of O15 antigen. PCR assays were developed for sensitive and specific detection of the O15-antigen-specific genes wzx and wzy. The high pathotype diversity found in the collection of 33 O15 strains contrasted with the high level of similarity found in the genes specific to the O15 antigen. This might indicate that the O15 determinant has been spread by horizontal gene transfer to a number of genetically unrelated strains of E. coli.

* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hong Da St., TEDA, Tianjin 300457, People's Republic of China. Phone: 86-22-66229592. Fax: 86-22-66229596. E-mail for Lei Wang: wanglei{at}nankai.edu.cn. E-mail for Lu Feng: fenglu63{at}nankai.edu.cn.

Journal of Clinical Microbiology, February 2005, p. 703-710, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.703-710.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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