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Journal of Clinical Microbiology, February 2005, p. 796-801, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.796-801.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic Variants of Anaplasma phagocytophilum Infecting Dogs in Western Washington State

Florence M. Poitout,1* Joanne K. Shinozaki,1 Patrick J. Stockwell,2 Cynthia J. Holland,3 and Sanjay K. Shukla2

Phoenix Central Laboratory for Veterinarians, Everett, Washington,1 Clinical Research Center, Marshfield Clinical Research Foundation, Marshfield, Wisconsin,2 Protatek Reference Laboratory, Chandler, Arizona3

Received 7 July 2004/ Returned for modification 7 August 2004/ Accepted 17 October 2004

Eight dogs from western Washington State suspected of being infected with Anaplasma phagocytophilum because of the finding of morulae in peripheral blood neutrophils were studied for determination of the etiologic agent of disease. All cases were diagnosed between April 2003 and April 2004. Six of the eight dogs had no travel history during the 6 months prior to presentation. Two dogs had traveled within the Northwest United States and Canada. Fever, lethargy, and anorexia were the most common clinical signs in the dogs. Lymphopenia, thrombocytopenia, and an elevated activity of alkaline phosphatase in the serum were the most common laboratory findings. All dogs tested during the acute phase of clinical signs were seropositive for A. phagocytophilum antibodies but negative for Ehrlichia canis antibodies. PCR amplification and direct sequencing of portions of the 16S rRNA gene from the whole blood of all seven dogs that were tested yielded A. phagocytophilum after a comparison to bacterial sequences available in the GenBank database. Five genetic variants were identified based on one or two nucleotide differences in the 16S rRNA gene sequences at nucleotide positions 54, 84, 86, and 120. Individual dogs were infected with more than one variant. Treatment with doxycycline or tetracycline resulted in a rapid resolution of clinical signs. The occurrence of canine granulocytic anaplasmosis in western Washington State suggests that A. phagocytophilum infection should be considered in differential diagnoses of dogs presenting with lethargy, anorexia, fever, and lameness, particularly in the context of lymphopenia, thrombocytopenia, and increased serum alkaline phosphatase. The zoonotic importance of A. phagocytophilum should support an increase in surveillance for horses and people residing in this area.

* Corresponding author. Mailing address: Phoenix Central Laboratory, 11620 Airport Rd., Everett, WA 98204-3742. Phone: (425) 355- 8787. Fax: (425) 355 3676. E-mail: poitout{at}msn.com.

Journal of Clinical Microbiology, February 2005, p. 796-801, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.796-801.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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