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Journal of Clinical Microbiology, February 2005, p. 808-812, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.808-812.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT)-PCR and TaqMan RT-PCR

Manfred Weidmann,1* P. Schmidt,1 M. Vackova,2 K. Krivanec,3 P. Munclinger,4 and F. T. Hufert1

Unit for Microbiological Preparedness and Emerging Infectious Diseases, University Hospital, Freiburg, Germany,1 Purkyne Military Medical Academy, Department of Epidemiology, Hradec Kralove,2 Central Military Institute of Health, Ceske Budejovice,3 Charles University Prague, Biodiversity Research Group, Department of Zoology, Prague, Czech Republic4

Received 12 May 2004/ Returned for modification 23 July 2004/ Accepted 6 September 2004

A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.

* Corresponding author. Mailing address: Unit for Microbiological Preparedness and Emerging Infectious Diseases, University Hospital, Stefan-Meier-Str. 19, 79014 Freiburg, Germany. Phone 49 761 203 5436. Fax: 49 761 203 5434. E-mail: manfred.weidmann{at}uniklinik-freiburg.de.

Journal of Clinical Microbiology, February 2005, p. 808-812, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.808-812.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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