This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, X.
Right arrow Articles by Lu, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, X.
Right arrow Articles by Lu, Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2005, p. 826-831, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.826-831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Outbreak of Infection Caused by Enterobacter cloacae Producing the Novel VEB-3 Beta-Lactamase in China

Xiaofei Jiang,1 Yuxing Ni,2 Yanqun Jiang,3 Feiyi Yuan,1 Lizhong Han,2 Ming Li,1 Hong Liu,1 Li Yang,2 and Yuan Lu1*

Center of Laboratory Medicine of Huashan Hospital, Shanghai Medical College, Fudan University,1 Department of Clinical Microbiology, Rijin Hospital, Shanghai Second Medical University,2 Department of Clinical Laboratory of the Sixth People's Hospital of Shanghai, Shanghai, People's Republic of China3

Received 15 April 2004/ Returned for modification 10 August 2004/ Accepted 12 October 2004

Over a 4-month period from November 2002 to February 2003, 27 ceftazidime-resistant or cefotaxime-resistant nonrepetitive Enterobacter cloacae isolates were collected from 27 patients hospitalized at HuaShan Hospital, Shanghai, People's Republic of China. The Etest did not detect extended-spectrum beta-lactamases (ESBLs) in those 27 isolates; however, screening by the NCCLS ESBL disk test and confirmatory tests detected ESBLs in 4 of 27 isolates and PCR detected ESBLs in 23 of 27 isolates. The majority of ESBL producers exhibited the same repetitive extragenic palindromic PCR pattern but harbored different ESBL genes. CTX-M-3 was the most prevalent ESBL in our study. Interestingly, 12 clonally related E. cloacae isolates possessed a novel blaVEB-type beta-lactamase, blaVEB-3. BlaVEB-3 was encoded by the chromosome and was located in an integron. Nine of the 12 isolates harbored both the blaVEB-3 and the blaCTX-M-3-like ESBLs. This is the first report of a VEB-1-like ESBL in China and the first report of the simultaneous presence of VEB-1 and CTX-M-3-like ESBLs in an isolate.

* Corresponding author. Mailing address: Center of Laboratory Medicine of Huashan Hospital, Fudan University, 12 Central Urumqi Rd., Shanghai 200040, People's Republic of China. Phone: 86 021 62489999 6430. Fax: 86-021-62481061. E-mail: yuanlu{at}hsh.stn.sh.cn.

Journal of Clinical Microbiology, February 2005, p. 826-831, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.826-831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zong, Z., Partridge, S. R., Iredell, J. R. (2009). A blaVEB-1 Variant, blaVEB-6, Associated with Repeated Elements in a Complex Genetic Structure. Antimicrob. Agents Chemother. 53: 1693-1697 [Abstract] [Full Text]  
  • Wu, Q., Zhang, Y., Han, L., Sun, J., Ni, Y. (2009). Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China. Antimicrob. Agents Chemother. 53: 271-272 [Abstract] [Full Text]  
  • Li, Y., Li, Q., Du, Y., Jiang, X., Tang, J., Wang, J., Li, G., Jiang, Y. (2008). Prevalence of Plasmid-Mediated AmpC {beta}-Lactamases in a Chinese University Hospital from 2003 to 2005: First Report of CMY-2-Type AmpC {beta}-Lactamase Resistance in China. J. Clin. Microbiol. 46: 1317-1321 [Abstract] [Full Text]  
  • Manzur, A., Tubau, F., Pujol, M., Calatayud, L., Dominguez, M. A., Pena, C., Sora, M., Gudiol, F., Ariza, J. (2007). Nosocomial Outbreak Due to Extended-Spectrum-Beta-Lactamase- Producing Enterobacter cloacae in a Cardiothoracic Intensive Care Unit. J. Clin. Microbiol. 45: 2365-2369 [Abstract] [Full Text]  
  • Paauw, A., Verhoef, J., Fluit, A. C., Blok, H. E. M., Hopmans, T. E. M., Troelstra, A., Leverstein-van Hall, M. A. (2007). Failure To Control an Outbreak of qnrA1-Positive Multidrug-Resistant Enterobacter cloacae Infection despite Adequate Implementation of Recommended Infection Control Measures. J. Clin. Microbiol. 45: 1420-1425 [Abstract] [Full Text]  
  • Jiang, X., Zhang, Z., Li, M., Zhou, D., Ruan, F., Lu, Y. (2006). Detection of Extended-Spectrum {beta}-Lactamases in Clinical Isolates of Pseudomonas aeruginosa.. Antimicrob. Agents Chemother. 50: 2990-2995 [Abstract] [Full Text]  
  • Toleman, M. A., Bennett, P. M., Walsh, T. R. (2006). ISCR Elements: Novel Gene-Capturing Systems of the 21st Century?. Microbiol. Mol. Biol. Rev. 70: 296-316 [Abstract] [Full Text]  
  • Naas, T., Aubert, D., Lambert, T., Nordmann, P. (2006). Complex Genetic Structures with Repeated Elements, a sul-Type Class 1 Integron, and the blaVEB Extended-Spectrum {beta}-Lactamase Gene.. Antimicrob. Agents Chemother. 50: 1745-1752 [Abstract] [Full Text]  
  • Pancer, K., Gut, W., Fila, S., Trzcinska, A., Roszkowiak, A., Laudy, A. E., Wernik, T., Mikulak, E., Gliniewicz, A., Stypulkowska-Misiurewicz, H. (2006). Strains of Enterobacter cloacae Isolated in a Hospital Environment - Some Phenotypic and Genotypic Properties. Indoor and Built Environment 15: 99-104 [Abstract]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Aubert, D., Naas, T., Lartigue, M.-F., Nordmann, P. (2005). Novel Genetic Structure Associated with an Extended-Spectrum {beta}-Lactamase blaVEB Gene in a Providencia stuartii Clinical Isolate from Algeria. Antimicrob. Agents Chemother. 49: 3590-3592 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»