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Journal of Clinical Microbiology, February 2005, p. 906-908, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.906-908.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Aspergillus fumigatus and a Mutation That Confers Reduced Susceptibility to Itraconazole and Posaconazole by Real-Time PCR and Pyrosequencing

Jason P. Trama, Eli Mordechai, and Martin E. Adelson*

Medical Diagnostic Laboratories, L.L.C., Hamilton, New Jersey

Received 21 July 2004/ Returned for modification 2 September 2004/ Accepted 30 September 2004

A real-time PCR and pyrosequencing method was developed to detect Aspergillus fumigatus in whole blood by amplifying the cyp51A gene and sequencing the codon for glycine 54. Mutations in this codon can result in amino acid substitutions that confer reduced susceptibility to itraconazole and posaconazole.

* Corresponding author. Mailing address: Medical Diagnostic Laboratories, L.L.C. 2439 Kuser Rd., Hamilton, NJ 08690. Phone: (609) 570-1015. Fax: (609) 570-1030. E-mail: madelson{at}mdlab.com.

Journal of Clinical Microbiology, February 2005, p. 906-908, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.906-908.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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