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Journal of Clinical Microbiology, February 2005, p. 925-927, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.925-927.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Quality Control Guidelines for Testing Gram-Negative Control Strains with Polymyxin B and Colistin (Polymyxin E) by Standardized Methods

Ronald N. Jones,1,2* Tamara R. Anderegg,1,3 Jana M. Swenson,3 and The Quality Control Working Group{dagger}

The JONES Group/JMI Laboratories, North Liberty, Iowa,1 Tufts University School of Medicine, Boston, Massachusetts,2 Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 7 September 2004/ Returned for modification 19 October 2004/ Accepted 22 October 2004

An eight-laboratory study addressed the urgent need for quality control (QC) ranges for susceptibility determination when testing colistin (polymyxin E) and polymyxin B, two polycationic peptide antimicrobial agents, against multidrug-resistant gram-negative bacilli. For Escherichia coli ATCC 25922l, the QC ranges were as follows: for colistin, 0.25 to 1 µg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 µg/ml (13 to 19 mm). For Pseudomonas aeruginosa ATCC 27853, the QC ranges were as follows: for colistin, 0.25 to 2 µg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 µg/ml (14 to 18 mm). More than 97% of all reported QC results were within these proposed ranges.


* Corresponding author. Mailing address: The JONES Group/JMI Laboratories, Inc., 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317. Phone: (319) 665-3370. Fax: (319) 665-3371. E-mail: ronald-jones{at}jmilabs.com.

{dagger} Members of the Quality Control Working Group are listed in Acknowledgments.


Journal of Clinical Microbiology, February 2005, p. 925-927, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.925-927.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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