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Journal of Clinical Microbiology, February 2005, p. 931-932, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.931-932.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the Restriction Enzyme EcoRI for Pulsed-Field Gel Electrophoretic Analysis of Helicobacter pylori

Yoshio Hosaka,1,2 Kazuhiko Irinoda,1 Ryuichi Nakano,1 Satosi Tanabe,2 Wasaburo Koizumi,2 Katunori Saigenji,2 and Matsuhisa Inoue1*

Department of Microbiology,1 First Department of Internal Medicine, Kitasato University School of Medicine, Kanagawa, Japan2

Received 2 May 2004/ Returned for modification 13 June 2004/ Accepted 6 October 2004

Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).

* Corresponding author. Mailing address: Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228, Japan. Phone: 81 42 778 9349. Fax: 81 42 778 9350. E-mail: matsu{at}kitasato-u.ac.jp.

Journal of Clinical Microbiology, February 2005, p. 931-932, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.931-932.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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