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Journal of Clinical Microbiology, March 2005, p. 1072-1075, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1072-1075.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Extended-Spectrum ß-Lactamase-Producing Shiga Toxin Gene (stx1)-Positive Escherichia coli O26:H11: a New Concern

Yoshikazu Ishii,1* Soichiro Kimura,1 Jimena Alba,1 Katsuaki Shiroto,1 Masanobu Otsuka,2 Naotaka Hashizume,2 Kazumichi Tamura,3 and Keizo Yamaguchi1

Department of Microbiology, Toho University School of Medicine,1 Department of Clinical Laboratory, Toho University Ohashi Hospital,2 Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan3

Received 4 August 2004/ Returned for modification 24 September 2004/ Accepted 3 October 2004

Escherichia coli strain TUM2139 was isolated from a stool sample from a 9-year-old girl on 16 June 2004. This strain was categorized as Shiga toxin-producing Escherichia coli (STEC) because the Shiga-like toxin gene stx1 was detected by immunochromatography and PCR assay. The strain was highly resistant to cefotaxime (256 µg/ml) and was also resistant to cefepime, cefpodoxime, ceftriaxone, and aztreonam. In the presence of 4 µg of clavulanic acid per ml, the MIC of cefotaxime decreased to ≤0.12 µg/ml, indicating that this strain was an extended-spectrum ß-lactamase (ESBL) producer. Cefotaxime resistance was transferred to E. coli C600 by conjugation at a frequency of 3.0 x 10–6. A PCR assay was performed with primer sets specific for TEM-type and SHV-type ESBLs and for the CTX-M-2 (Toho-1), CTX-M-3, and CTX-M-9 groups of ESBLs. A specific signal was observed with the primer set specific for the CTX-M-9 group of ß-lactamases. This ß-lactamase was confirmed to be the ESBL CTX-M-18 by DNA sequencing. This is the first report of an ESBL-producing STEC isolate.

* Corresponding author. Mailing address: Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 1438540 Tokyo, Japan. Phone: 81-3-3762-4151. Fax: 81-3-5493-5415. E-mail: yoishii{at}med.toho-u.ac.jp.

Journal of Clinical Microbiology, March 2005, p. 1072-1075, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1072-1075.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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