JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fischer, T. K.
Right arrow Articles by Nielsen, N. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fischer, T. K.
Right arrow Articles by Nielsen, N. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2005, p. 1099-1104, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1099-1104.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Rotavirus Strains in a Danish Population: High Frequency of Mixed Infections and Diversity within the VP4 Gene of P[8] Strains

T. K. Fischer,1,2* J. Eugen-Olsen,3 A. G. Pedersen,4 K. Mølbak,5 B. Böttiger,6 K. Rostgaard,2 and N. M. Nielsen2,3

Division of Rickettsial and Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,1 Department of Epidemiology Research,2 Department of Epidemiology,5 Department of Virology, Statens Serum Institut,6 Clinical Research Unit, Hvidovre Hospital, Copenhagen,3 Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark4

Received 13 August 2004/ Returned for modification 26 October 2004/ Accepted 14 November 2004

We characterized the G and P types from 162 rotavirus-positive stool specimens collected from 162 persons in Denmark (134 children and 28 adults) with acute diarrhea in 1998, 2000, and 2002. Samples were obtained during outpatient consultations (73%) and from hospitalized patients (27%). Although more than 20 different G-P combinations were identified, only 52% represented the globally most common types G1P[8], G2P[4], and G4P[8]. The G9 genotype, which is emerging worldwide, was identified in 12% of all samples. Twenty-one percent of the samples were of mixed genotypic origin, which is the highest frequency reported in any European population. The standard reverse transcription-PCR methods initially failed to identify a considerable fraction of the rotavirus P strains due to mutations at the VP4 primer-binding sites of P[8] strains. The application of a degenerate P[8] primer resulted in typing of most VP4 strains. There was considerable year-to-year variation among the circulating G-P types, and whereas G1P[8] was predominant in 1998 (42% of samples) and 2002 (26%), G2P[4] was the strain that was most frequently detected in 2000 (26% of samples). Our findings might implicate challenges for rotavirus vaccine implementation in a European population and underscore the importance of extensive strain surveillance prior to, during, and after introduction of any vaccine candidate.

* Corresponding author. Mailing address: Viral Gastroenteritis Section, MS G-04, Centers for Disease Control and Prevention, 1630 Clifton Rd., N.E., Atlanta, GA 30333. Phone: (404) 639-3577. Fax: (404) 639-3645. E-mail: thf{at}ssi.dk.

Journal of Clinical Microbiology, March 2005, p. 1099-1104, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1099-1104.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2005 by the American Society for Microbiology. All rights reserved.