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Journal of Clinical Microbiology, March 2005, p. 1366-1371, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1366-1371.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Predominance of afr2 and ral Fimbrial Genes Related to Those Encoding the K88 and CS31A Fimbrial Adhesins in Enteropathogenic Escherichia coli Isolates from Rabbits with Postweaning Diarrhea in Central Europe

Mohamed A. Dow,1 István Tóth,1 Pavel Alexa,2 Michael Davies,3 Anna Malik,1 Eric Oswald,4 and Béla Nagy1*

Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary,1 Veterinary Research Institute, Brno, Czech Republic,2 Gastroenteric Disease Center, The Pennsylvania State University, University Park, Pennsylvania,3 UMR 1225 INRA "Interactions Hotes-Agents Pathogénes," Ecole Nationale Veterinaire de Toulouse, Toulouse, France4

Received 30 December 2003/ Returned for modification 20 May 2004/ Accepted 7 November 2004

PCR tests designed in these studies identified three rabbit adhesive factor genes among 43 enteropathogenic E. coli (EPEC) strains: afr1 (2 strains), the F4(K88)/CS31A-related afr2 (10 strains), and ral (15 strains). Several EPEC strains (i.e., O153:H7 and O157:H2) lacked these genes but did adhere to HeLa cells and produced attaching and effacing lesions in rabbits.


* Corresponding author. Mailing address: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, 1143 Budapest, Hungaria Str. 21, Budapest, Hungary. Phone: 36-1-2522455. Fax: 36-1-2521069. E-mail: bnagy{at}vmri.hu.


Journal of Clinical Microbiology, March 2005, p. 1366-1371, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1366-1371.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.







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