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Journal of Clinical Microbiology, March 2005, p. 1387-1389, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1387-1389.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Microsatellite Markers and Gene Dosage To Quantify Gene Copy Numbers in Candida albicans

J.-M. Costa,1,2 O. Eloy,3 F. Botterel,1 G. Janbon,4 and S. Bretagne1*

Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-APHP and UMR BIPAR 956, Créteil,1 Laboratoire de Biologie Moléculaire, Hôpital Américain, Neuilly,2 Laboratoire de Parasitologie, Hôpital Mignot, Versailles,3 Unité de Mycologie Moléculaire, Institut Pasteur, Paris, France4

Received 2 April 2004/ Returned for modification 22 April 2004/ Accepted 19 October 2004

With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital H. Mondor, 51 avenue du Maréchal DeLattre de Tassigny, 94010 Créteil, France. Phone: 33 1 49 81 36 41. Fax: 33 1 49 81 36 01. E-mail: bretagne{at}univ-paris12.fr.

Journal of Clinical Microbiology, March 2005, p. 1387-1389, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1387-1389.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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