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Journal of Clinical Microbiology, March 2005, p. 1411-1414, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1411-1414.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Human Metapneumovirus RNA Sequences in Nasopharyngeal Aspirates of Young French Children with Acute Bronchiolitis by Real-Time Reverse Transcriptase PCR and Phylogenetic Analysis

Maude Bouscambert-Duchamp,1,2 Bruno Lina,1 Aurélien Trompette,1 Hélène Moret,2 Jacques Motte,3 and Laurent Andréoletti2*

Laboratoire de Virologie des Hospices Civils de Lyon, Lyon,1 Laboratoire de Virologie Hôpital Robert Debré, CHU et ER-3798 (IFR 53) Faculté de Médecine de Reims,2 Service des Urgences Médico-chirurgicales Pédiatriques et ER-3798 (IFR 53) Faculté de Médecine de Reims, Champagne-Ardenne, France3

Received 27 July 2004/ Returned for modification 27 September 2004/ Accepted 23 October 2004

Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, after respiratory syncytial virus and rhinovirus (35 [37%] and 21 [22%] of 94 cases, respectively). Phylogenetic analysis of F-gene sequences demonstrated the cocirculation of distinct HMPV genotypes during this study. These findings highlight the need to implement a rapid HMPV RT-PCR detection assay for the clinical diagnosis of respiratory infections in pediatric patients with bronchiolitis.

* Corresponding author. Mailing address: Laboratoire de Virologie, Service de Microbiologie, Hôpital Robert Debré, Avenue du Général Koenig, 51092 REIMS Cedex, France. Phone: (33) 3 26 78 39 93. Fax: (33) 3 26 78 41 34. E-mail: landreoletti{at}chu-reims.fr.

Journal of Clinical Microbiology, March 2005, p. 1411-1414, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1411-1414.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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