This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robberts, F. J. L.
Right arrow Articles by Liebowitz, L. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robberts, F. J. L.
Right arrow Articles by Liebowitz, L. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2005, p. 1443-1444, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1443-1444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Dihydropteroate Synthase and Novel Dihydrofolate Reductase Gene Mutations in Strains of Pneumocystis jirovecii from South Africa

F. J. L. Robberts,1* L. J. Chalkley,2 K. Weyer,3 P. Goussard,4 and L. D. Liebowitz1

Department of Medical Microbiology, University of Stellenbosch,1 Department of Pediatrics and Child Health, Tygerberg Hospital, Tygerberg,4 MRC-SA, Parow,2 MRC-SA TB Operational and Policy Research Unit, Pretoria, South Africa3

Received 19 September 2004/ Returned for modification 27 October 2004/ Accepted 14 November 2004

Dihydropteroate synthase (DHPS) gene mutations have raised concerns about emerging sulfonamide resistance in Pneumocystis jirovecii. DHPS and dihydrofolate reductase (DHFR) gene products were amplified in clinical specimens from South African patients. One of 53 DHPS genes sequenced contained the double mutation Thr55Ala Pro57Ser. DHFR gene mutations detected were Ala67Val and the new mutations Arg59Gly and C278T.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Stellenbosch, Tygerberg 7500, South Africa. Phone: 021 938 5228. Fax: 011 27 021 9384005. E-mail: lourensr{at}sun.ac.za.

Journal of Clinical Microbiology, March 2005, p. 1443-1444, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1443-1444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Costa, M. C., Esteves, F., Antunes, F., Matos, O. (2006). Genetic characterization of the dihydrofolate reductase gene of Pneumocystis jirovecii isolates from Portugal. J Antimicrob Chemother 58: 1246-1249 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»