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Journal of Clinical Microbiology, March 2005, p. 1445-1447, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1445-1447.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, Including Confirmation with N. gonorrhoeae-Specific 16S rRNA PCR, with Traditional Culture

Dirk S. Luijt,1 Petra A. J. Bos,1 Anton A. van Zwet,1 Pieter C. van Voorst Vader,2 and Jurjen Schirm1*

Regional Public Health Laboratory,1 Department of Dermatology, University Hospital Groningen, Groningen, The Netherlands2

Received 28 May 2004/ Returned for modification 23 August 2004/ Accepted 22 October 2004

A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.

* Corresponding author. Mailing address: Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands. Phone: 31-50-5215160. Fax: 31-50-5271488. E-mail: j.schirm{at}slgd.nl.

Journal of Clinical Microbiology, March 2005, p. 1445-1447, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1445-1447.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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