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Journal of Clinical Microbiology, April 2005, p. 1917-1920, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1917-1920.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

D. A. Santos and J. S. Hamdan*

Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Received 23 September 2004/ Returned for modification 5 November 2004/ Accepted 29 November 2004

Fifty clinical isolates of Trichophyton rubrum were selected to test with ketoconazole, fluconazole, itraconazole, griseofulvin, and terbinafine by following the National Committee for Clinical Laboratory Standards susceptibility testing guidelines for filamentous fungi (M38-A). In addition, other susceptibility testing conditions were evaluated: (i) three medium formulations including RPMI 1640 (standard medium), McVeigh & Morton (MVM), and Sabouraud dextrose broth (SDB); (ii) two incubation temperatures (28 and 35°C); and (iii) three incubation periods (4, 7, and 10 days). The strains Candida parapsilosis (ATCC 22019), Candida krusei (ATCC 6258), T. rubrum (ATCC 40051), and Trichophyton mentagrophytes (ATCC 40004) were included as quality controls. All isolates produced clearly detectable growth only after 7 days of incubation. MICs were significantly independent of the incubation temperature (28 or 35°C) (P < 0.05). Different incubation periods resulted in MICs which were consistently different for each medium when azoles and griseofulvin were tested (P < 0.05). MICs obtained from different media at the same incubation time for the same isolate were significantly different when azoles and griseofulvin were tested (P < 0.05). MICs were consistently higher (usually 1 to 2 dilutions) with RPMI than with MVM or SDB (P < 0.05). When terbinafine was tested, no parameter had any influence on MICs (P < 0.05). RPMI standard medium appears to be a suitable testing medium for determining the MICs for T. rubrum. MICs obtained at different incubation times need to be correlated with clinical outcome to demonstrate which time has better reliability.

* Corresponding author. Mailing address: Universidade Federal de Minas Gerais, Departamento de Microbiologia, Av. Antônio Carlos, 6627, P. O. Box 486, Belo Horizonte, Minas Gerais, Brazil. Phone: 5531 499 2758. Fax: 5531 499 2730. E-mail: handan{at}mono.icb.ufmg.br.

Journal of Clinical Microbiology, April 2005, p. 1917-1920, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1917-1920.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Barros, M. E. d. S., Santos, D. d. A., Hamdan, J. S. (2007). Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A). J Med Microbiol 56: 514-518 [Abstract] [Full Text]  
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