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Journal of Clinical Microbiology, April 2005, p. 1921-1924, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1921-1924.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Direct Detection of Nocardia spp. in Clinical Samples by a Rapid Molecular Method

Andrée Couble,1 Verónica Rodríguez-Nava,1 Michèle Pérouse de Montclos,2 Patrick Boiron,1 and Frédéric Laurent1*

UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Faculté de Pharmacie, Université Claude Bernard Lyon I, Lyon,1 Laboratoire de Bactériologie, Centre Hospitalier Lyon Sud, Pierre Bénite, France2

Received 13 October 2004/ Returned for modification 24 November 2004/ Accepted 6 December 2004

We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.

* Corresponding author. Mailing address: Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, UMR CNRS 5557, Faculté de Pharmacie, UCB Lyon I, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France. Phone: (33) (0) 4 78 77 70 00. Fax: (33) (0) 4 78 77 72 12. E-mail: flaurent{at}sante.univ-lyon1.fr.

Journal of Clinical Microbiology, April 2005, p. 1921-1924, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1921-1924.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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