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Journal of Clinical Microbiology, April 2005, p. 1930-1931, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1930-1931.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar

Ahmet Yilmaz Coban,1* Kemal Bilgin,1 Meltem Uzun,2 Nuriye Tasdelen Fisgin,3 Alper Akgunes,1 Cigdem Cekic Cihan,1 Asuman Birinci,1 and Belma Durupinar1

Department of Microbiology and Clinical Microbiology,1 Department of Clinical Microbiology and Infectious Diseases, Medical School, Ondokuz, Mayis University, Samsun,3 Department of Microbiology and Clinical Microbiology, Istanbul Medical School, Istanbul University, Istanbul, Turkey2

Received 4 August 2004/ Returned for modification 15 September 2004/ Accepted 15 November 2004

In this study, blood agar was used instead of 7H10 agar for the susceptibility testing of 34 clinical isolates of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) in accordance with the NCCLS. The BACTEC 460 TB system (Becton Dickinson, Sparks, Md.) was used as a "gold standard." Results for both media were in agreement for RIF and INH at 100 and 94.1%, respectively. For INH, the specificity, sensitivity, positive predictive value, and negative predictive value were found to be 71.4, 100, 93.1, and 100%, respectively, while these values were 100% for RIF. In addition, the results of the susceptibility test performed with blood agar were obtained on day 14 of incubation. In conclusion, results were obtained much earlier with blood agar (2 weeks) than with 7H10 agar (3 weeks), and the results of this study suggest that blood agar may be used as an alternative medium for the susceptibility testing of M. tuberculosis to INH and RIF.

* Corresponding author. Mailing address: Ondokuz Mayis University, Medical School, Department of Microbiology and Clinical Microbiology, 55139 Samsun, Turkey. Phone: 90 362 4576000, ext. 3526. Fax: 90 362 4576041. E-mail: noahmet{at}yahoo.com.

Journal of Clinical Microbiology, April 2005, p. 1930-1931, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1930-1931.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Coban, A. Y., Bilgin, K., Uzun, M., Akgunes, A., Yusof, A., Durupinar, B. (2008). Comparative Study for Determination of Mycobacterium tuberculosis Susceptibility to First- and Second-Line Antituberculosis Drugs by the Etest Using 7H11, Blood, and Chocolate Agar. J. Clin. Microbiol. 46: 4095-4098 [Abstract] [Full Text]  


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