Previous Article | Next Article 
Journal of Clinical Microbiology, April 2005, p. 1934-1936, Vol. 43, No. 4
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.4.1934-1936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Comparison between rpoB and 16S rRNA Gene Sequencing for Molecular Identification of 168 Clinical Isolates of Corynebacterium
Atieh Khamis,
Didier Raoult, and
Bernard La Scola*
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerrannée, Marseille, France
Received 4 August 2004/
Returned for modification 6 October 2004/
Accepted 16 December 2004
Higher proportions (91%) of 168 corynebacterial isolates were positively identified by partial rpoB gene determination than by that based on 16S rRNA gene sequences. This method is thus a simple, molecular-analysis-based method for identification of corynebacteria, but it should be used in conjunction with other tests for definitive identification.
* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerrannée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33.04.91.38.55.17. Fax: 33.04.91.83.03.90. E-mail: bernard.lascola{at}medecine.univ-mrs.fr.
Journal of Clinical Microbiology, April 2005, p. 1934-1936, Vol. 43, No. 4
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.4.1934-1936.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Adderson, E. E., Boudreaux, J. W., Cummings, J. R., Pounds, S., Wilson, D. A., Procop, G. W., Hayden, R. T.
(2008). Identification of Clinical Coryneform Bacterial Isolates: Comparison of Biochemical Methods and Sequence Analysis of 16S rRNA and rpoB Genes. J. Clin. Microbiol.
46: 921-927
[Abstract]
[Full Text]
-
Renaud, F. N. R., Coustumier, A. L., Wilhem, N., Aubel, D., Riegel, P., Bollet, C., Freney, J.
(2007). Corynebacterium hansenii sp. nov., an {alpha}-glucosidase-negative bacterium related to Corynebacterium xerosis. Int. J. Syst. Evol. Microbiol.
57: 1113-1116
[Abstract]
[Full Text]
-
Pacheco, L. G. C., Pena, R. R., Castro, T. L. P., Dorella, F. A., Bahia, R. C., Carminati, R., Frota, M. N. L., Oliveira, S. C., Meyer, R., Alves, F. S. F., Miyoshi, A., Azevedo, V.
(2007). Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. J Med Microbiol
56: 480-486
[Abstract]
[Full Text]
-
Tarr, C. L., Patel, J. S., Puhr, N. D., Sowers, E. G., Bopp, C. A., Strockbine, N. A.
(2007). Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination. J. Clin. Microbiol.
45: 134-140
[Abstract]
[Full Text]
Copyright © 2005 by the American Society for Microbiology. All rights reserved.