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Journal of Clinical Microbiology, April 2005, p. 1940-1943, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1940-1943.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Typing Strains of Toxoplasma gondii

Daniel Ajzenberg, Aurélien Dumètre, and Marie-Laure Dardé*

Laboratoire de Parasitologie-Mycologie, Faculté de Médecine EA 3174, Limoges, France

Received 3 September 2004/ Returned for modification 22 October 2004/ Accepted 30 November 2004

A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.

* Corresponding author. Mailing address: Department of Parasitology and Mycology, CHU Dupuytren, 87042 Limoges cedex, France. Phone: 33-5-55-05-61-60. Fax: 33-5-55-05-61-77. E-mail: darde{at}unilim.fr.

Journal of Clinical Microbiology, April 2005, p. 1940-1943, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1940-1943.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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