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Journal of Clinical Microbiology, April 2005, p. 1944-1946, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1944-1946.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Improvement of the Specificity of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Paracoccidioidomycosis

C. F. Albuquerque, S. H. Marques da Silva, and Z. P. Camargo*

Department of Microbiology, Immunology and Parasitology, Cellular Biology Discipline, Federal University of São Paulo, São Paulo, Brazil

Received 17 August 2004/ Returned for modification 13 October 2004/ Accepted 16 December 2004

In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. The maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.

* Corresponding author. Mailing address: Universidade Federal de São Paulo/EPM, Departamento de Microbiologia, Imunologia e Parasitologia, Disciplina de Biologia Celular, 04023-062 Rua Botucatu, 862, São Paulo, S.P., Brazil. Phone: 55 11 5576 45 23. Fax: 55 11 5571 58 77. E-mail: zoilo{at}ecb.epm.br.

Journal of Clinical Microbiology, April 2005, p. 1944-1946, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1944-1946.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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