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Journal of Clinical Microbiology, April 2005, p. 1950-1952, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1950-1952.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines

Marie L. Landry,1* David Ferguson,1 Sandra Cohen,1 Teresa C. T. Peret,2 and Dean D. Erdman2

Department of Laboratory Medicine, Yale New Haven Hospital and Yale University School of Medicine, New Haven, Connecticut,1 Respiratory and Enteric Viruses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 7 June 2004/ Returned for modification 9 August 2004/ Accepted 23 November 2004

Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Yale University School of Medicine, P.O. Box 208035, New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu.

Journal of Clinical Microbiology, April 2005, p. 1950-1952, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1950-1952.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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