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Journal of Clinical Microbiology, April 2005, p. 1953-1955, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1953-1955.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Latex Agglutination Test for Monitoring Antibodies to Avian Influenza Virus Subtype H5N1

Xiaojuan Xu,1 Meilin Jin,1,2* Zhengjun Yu,1 Hongchao Li,1 Dexin Qiu,1 Yadi Tan,1,2 and Huanchun Chen1,2

Laboratory of Animal Virology, College of Veterinary Medicine,1 Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China2

Received 12 August 2004/ Returned for modification 6 November 2004/ Accepted 16 December 2004

A latex agglutination test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated chickens. The test has application potential in field practice.

* Corresponding author. Mailing address: Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Phone: 86-27-87282608. Fax: 86-27-87281795. E-mail: hzauvet{at}public.wh.hb.cn.

Journal of Clinical Microbiology, April 2005, p. 1953-1955, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1953-1955.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Love, D. C., Sobsey, M. D. (2007). Simple and Rapid F+ Coliphage Culture, Latex Agglutination, and Typing Assay To Detect and Source Track Fecal Contamination. Appl. Environ. Microbiol. 73: 4110-4118 [Abstract] [Full Text]  


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