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Journal of Clinical Microbiology, April 2005, p. 1963-1967, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1963-1967.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identity and Prevalence of Multilocus Sequence Typing-Defined Clones of Group A Streptococci within a Hospital Setting

Karen F. McGregor{dagger} and Brian G. Spratt*

Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, St. Mary's Hospital, London, United Kingdom

Received 18 October 2004/ Returned for modification 19 November 2004/ Accepted 30 November 2004

Between July and October 2003, 121 clinical isolates of group A streptococci (GAS) were collected from a London hospital and characterized by multilocus sequence typing (MLST) to determine the identity and prevalence of clones circulating within this setting. A total of 39 sequence types (ST), of which 20 were represented by a single isolate, were identified. The eight most prevalent clones among the 121 GAS were ST117/emm81 (16%), ST39/emm4 (9%), ST62/emm87 (7%), ST28/emm1 (6%), ST36/emm12 (6%), ST46/emm22 (5%), ST334/emm82 (5%), and ST101/emm89 (4%). Compared to those in the MLST database (http://spyogenes.mlst.net), 12 (31%) of the 39 STs had not been previously identified, although 7 of these differed from recognized STs at only a single locus, suggesting they were closely related to previously recognized strains. Resistance to erythromycin and tetracycline was seen in 7 and 20% of isolates, respectively, with four isolates resistant to both agents. GAS strains with higher (>80) emm types accounted for 45% of GAS isolates collected during this study. Continuing GAS surveillance, using easily comparable methods, is important for detecting changes in the character of disease-causing isolates.

* Corresponding author. Mailing address: Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, St. Mary's Hospital Medical School, Norfolk Place, London W2 1PG, United Kingdom. Phone: 44 207 594 3629. Fax: 44 207 594 3693. E-mail: b.spratt{at}imperial.ac.uk.

{dagger} Present address: Microbiology Research Group, Thames Valley University, London W5 5AA, United Kingdom.

Journal of Clinical Microbiology, April 2005, p. 1963-1967, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1963-1967.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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