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Journal of Clinical Microbiology, April 2005, p. 1968-1972, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1968-1972.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Heterogeneity among Strains of Diffusely Adherent Escherichia coli Isolated in Brazil

Lucia M. Lopes,1 Sandra H. Fabbricotti,1 Antonio J. P. Ferreira,2 Maria A. M. F. Kato,3 Jane Michalski,4 and Isabel C. A. Scaletsky1*

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo,1 Instituto Adolfo Lutz,3 Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil,2 Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland4

Received 11 May 2004/ Returned for modification 6 August 2004/ Accepted 16 December 2004

One hundred twelve diffusely adherent Escherichia coli strains isolated from children in a case control study were evaluated for virulence-associated characteristics, serotyping, antibiotic resistance, and plasmid profiles. Half of the strains hybridized with the probes for icuA (aerobactin) and fimH (type 1 pili); daaE (F1845 fimbriae), afa (afimbrial Dr adhesin), agg-3A (aggregative adhesion fimbria type III fimbriae), pap (P fimbriae), astA (EAST1 toxin), and shET1 (Shigella enterotoxin 1) sequences were present in <20% of the strains. The shET1 gene was noted most frequently in strains isolated from patients. A minority (7%) of the strains produced hemolysin or colicin or showed cytotoxic effects on Vero cells. Forty-five different serotypes were found. The majority (70%) of the strains presented multiple antibiotic resistance. Antibiotic resistance and diffuse adherence were located on the same conjugative plasmids. These results suggest that the transfer of these potential virulence markers could be important in the epidemiology of diffusely adherent E. coli.

* Corresponding author. Mailing address: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu, 862 -30 andar, São Paulo, SP, Brazil CEP 04023-062. Phone: 55-11-55764537. Fax: 55-11-55716504. E-mail: scaletsky{at}ecb.epm.br.

Journal of Clinical Microbiology, April 2005, p. 1968-1972, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1968-1972.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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