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Journal of Clinical Microbiology, April 2005, p. 1995-1997, Vol. 43, No. 4
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.4.1995-1997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis
W. Ryan Easterday,1,
Matthew N. Van Ert,1,
Tatum S. Simonson,1
David M. Wagner,1
Leo J. Kenefic,1
Christopher J. Allender,1 and
Paul Keim1,2*
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640,1
The Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ 850042
Received 7 September 2004/
Returned for modification 13 October 2004/
Accepted 27 October 2004
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
* Corresponding author. Mailing address: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640. Phone: (928) 523-1078. Fax: (928) 523-0639. E-mail: Paul.Keim{at}nau.edu.
W.R.E. and M.N.V. contributed equally to the research described in this paper.
Journal of Clinical Microbiology, April 2005, p. 1995-1997, Vol. 43, No. 4
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.4.1995-1997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.