This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Easterday, W. R. Articles by Keim, P. Search for Related Content PubMed PubMed Citation Articles by Easterday, W. R. Articles by Keim, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2005, p. 1995-1997, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1995-1997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis

W. Ryan Easterday,^1, Matthew N. Van Ert,^1, Tatum S. Simonson,¹ David M. Wagner,¹ Leo J. Kenefic,¹ Christopher J. Allender,¹ and Paul Keim^1,2*

Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640,¹ The Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ 85004²

Received 7 September 2004/ Returned for modification 13 October 2004/ Accepted 27 October 2004

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

W.R.E. and M.N.V. contributed equally to the research described in this paper.

This article has been cited by other articles:

• Scott, J. C., Koylass, M. S., Stubberfield, M. R., Whatmore, A. M. (2007). Multiplex Assay Based on Single-Nucleotide Polymorphisms for Rapid Identification of Brucella Isolates at the Species Level. Appl. Environ. Microbiol. 73: 7331-7337 [Abstract] [Full Text]  
• Carter, D. J., Cary, R. B. (2007). Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography. Nucleic Acids Res 35: e74-e74 [Abstract] [Full Text]  
• Van Ert, M. N., Easterday, W. R., Simonson, T. S., U'Ren, J. M., Pearson, T., Kenefic, L. J., Busch, J. D., Huynh, L. Y., Dukerich, M., Trim, C. B., Beaudry, J., Welty-Bernard, A., Read, T., Fraser, C. M., Ravel, J., Keim, P. (2007). Strain-Specific Single-Nucleotide Polymorphism Assays for the Bacillus anthracis Ames Strain. J. Clin. Microbiol. 45: 47-53 [Abstract] [Full Text]  
• Keim, P., Mock, M., Young, J., Koehler, T. M. (2006). The International Bacillus anthracis, B. cereus, and B. thuringiensis Conference, "Bacillus-ACT05".. J. Bacteriol. 188: 3433-3441 [Full Text]  
• Bongiorni, C., Stoessel, R., Shoemaker, D., Perego, M. (2006). Rap Phosphatase of Virulence Plasmid pXO1 Inhibits Bacillus anthracis Sporulation. J. Bacteriol. 188: 487-498 [Abstract] [Full Text]  
• Valjevac, S., Hilaire, V., Lisanti, O., Ramisse, F., Hernandez, E., Cavallo, J.-D., Pourcel, C., Vergnaud, G. (2005). Comparison of Minisatellite Polymorphisms in the Bacillus cereus Complex: a Simple Assay for Large-Scale Screening and Identification of Strains Most Closely Related to Bacillus anthracis. Appl. Environ. Microbiol. 71: 6613-6623 [Abstract] [Full Text]  
• U'Ren, J. M., Van Ert, M. N., Schupp, J. M., Easterday, W. R., Simonson, T. S., Okinaka, R. T., Pearson, T., Keim, P. (2005). Use of a Real-Time PCR TaqMan Assay for Rapid Identification and Differentiation of Burkholderia pseudomallei and Burkholderia mallei. J. Clin. Microbiol. 43: 5771-5774 [Abstract] [Full Text]