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Journal of Clinical Microbiology, April 2005, p. 1995-1997, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1995-1997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis

W. Ryan Easterday,1,{dagger} Matthew N. Van Ert,1,{dagger} Tatum S. Simonson,1 David M. Wagner,1 Leo J. Kenefic,1 Christopher J. Allender,1 and Paul Keim1,2*

Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640,1 The Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ 850042

Received 7 September 2004/ Returned for modification 13 October 2004/ Accepted 27 October 2004

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

* Corresponding author. Mailing address: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640. Phone: (928) 523-1078. Fax: (928) 523-0639. E-mail: Paul.Keim{at}nau.edu.

{dagger} W.R.E. and M.N.V. contributed equally to the research described in this paper.

Journal of Clinical Microbiology, April 2005, p. 1995-1997, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1995-1997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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