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Journal of Clinical Microbiology, April 2005, p. 2004-2008, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.2004-2008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Multilocus Sequence Typing Directly on DNA from Clinical Samples and a Cultured Isolate To Investigate Linked Fatal Pneumococcal Disease in Residents of a Shelter for Homeless Men

Andrew Birtles,1 Noel McCarthy,2 Carmen L. Sheppard,1 Harry Rutter,3 Malcolm Guiver,4 Elizabeth Haworth,2 and Robert C. George1*

Health Protection Agency, Respiratory and Systemic Infections Laboratory, Central Public Health Laboratory, London ,1 Health Protection Agency, South East Region,2 South East Public Health Observatory,3 Health Protection Agency, Meningococcal Reference Unit, North West Regional Laboratory, Manchester, United Kingdom.4

Received 24 July 2004/ Returned for modification 14 September 2004/ Accepted 17 December 2004

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

* Corresponding author. Mailing address: Respiratory and Systemic Infection Laboratory, CPHL, 61 Colindale Avenue, London NW9 5HT, United Kingdom. Phone: 44 20 8327 7222. Fax: 44 20 8205 6528. E-mail: robert.george{at}hpa.org.uk.

Journal of Clinical Microbiology, April 2005, p. 2004-2008, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.2004-2008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Copyright © 2005 by the American Society for Microbiology. All rights reserved.