This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Clark, C. G. Articles by Wang, G. Search for Related Content PubMed PubMed Citation Articles by Clark, C. G. Articles by Wang, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2005, p. 2080-2091, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2080-2091.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the Oxford Multilocus Sequence Typing Protocol and Sequencing of the Flagellin Short Variable Region To Characterize Isolates from a Large Outbreak of Waterborne Campylobacter sp. Strains in Walkerton, Ontario, Canada

Bacteriology and Enteric Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada/Agence de santé Publique du Canada, Winnipeg, Canada,¹ National Microbiology Laboratory, Public Health Agency of Canada/Agence de santé Publique du Canada, Winnipeg, Canada,² Science and Advisory Unit, Food Inspection Branch, Ontario Ministry of Agriculture and Food, Guelph, Ontario, Canada,³ Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada⁴

Received 28 July 2004/ Returned for modification 24 September 2004/ Accepted 20 January 2005

The Walkerton (Ontario, Canada) outbreak of waterborne Escherichia coli O157:H7 and Campylobacter jejuni was quite limited in both space and time, making it a good model for exploring the utility of different typing and subtyping methods for the characterization of relationships among isolates of these organisms. We have extended previous work with these organisms through analysis by the Oxford multilocus sequence typing (MLST) and the flagellin short variable region (fla-SVR) sequencing methods. Additional isolates not epidemiologically related to the Walkerton outbreak have also been included. Both sequencing methods identified and differentiated between Walkerton outbreak strains 1 and 2. When these strains were compared with isolates that were not part of the outbreak, the information produced by the fla-SVR method more often correlated with epidemiological findings than that produced by MLST, though both methods were required for optimal discrimination. The MLST data were more relevant in terms of the overall population structure of the organisms. Both mutation and recombination appeared to be responsible for generating diversity among the isolates tested.

This article has been cited by other articles:

• Barton, C., Ng, L.-K., Tyler, S. D., Clark, C. G. (2007). Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni. J. Clin. Microbiol. 45: 386-391 [Abstract] [Full Text]  
• Djordjevic, S. P., Unicomb, L. E., Adamson, P. J., Mickan, L., Rios, R., the Australian Campylobacter Subtyping Study Group, (2007). Clonal Complexes of Campylobacter jejuni Identified by Multilocus Sequence Typing Are Reliably Predicted by Restriction Fragment Length Polymorphism Analyses of the flaA Gene. J. Clin. Microbiol. 45: 102-108 [Abstract] [Full Text]  
• Price, E. P., Huygens, F., Giffard, P. M. (2006). Fingerprinting of Campylobacter jejuni by Using Resolution-Optimized Binary Gene Targets Derived from Comparative Genome Hybridization Studies. Appl. Environ. Microbiol. 72: 7793-7803 [Abstract] [Full Text]  
• Price, E. P., Thiruvenkataswamy, V., Mickan, L., Unicomb, L., Rios, R. E., Huygens, F., Giffard, P. M. (2006). Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing.. J Med Microbiol 55: 1061-1070 [Abstract] [Full Text]