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Journal of Clinical Microbiology, May 2005, p. 2080-2091, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2080-2091.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the Oxford Multilocus Sequence Typing Protocol and Sequencing of the Flagellin Short Variable Region To Characterize Isolates from a Large Outbreak of Waterborne Campylobacter sp. Strains in Walkerton, Ontario, Canada

Clifford G. Clark,1* Louis Bryden,1 Wilfred R. Cuff,2 Patricia L. Johnson,3 Frances Jamieson,4 Bruce Ciebin,4 and Gehua Wang1

Bacteriology and Enteric Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada/Agence de santé Publique du Canada, Winnipeg, Canada,1 National Microbiology Laboratory, Public Health Agency of Canada/Agence de santé Publique du Canada, Winnipeg, Canada,2 Science and Advisory Unit, Food Inspection Branch, Ontario Ministry of Agriculture and Food, Guelph, Ontario, Canada,3 Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada4

Received 28 July 2004/ Returned for modification 24 September 2004/ Accepted 20 January 2005

The Walkerton (Ontario, Canada) outbreak of waterborne Escherichia coli O157:H7 and Campylobacter jejuni was quite limited in both space and time, making it a good model for exploring the utility of different typing and subtyping methods for the characterization of relationships among isolates of these organisms. We have extended previous work with these organisms through analysis by the Oxford multilocus sequence typing (MLST) and the flagellin short variable region (fla-SVR) sequencing methods. Additional isolates not epidemiologically related to the Walkerton outbreak have also been included. Both sequencing methods identified and differentiated between Walkerton outbreak strains 1 and 2. When these strains were compared with isolates that were not part of the outbreak, the information produced by the fla-SVR method more often correlated with epidemiological findings than that produced by MLST, though both methods were required for optimal discrimination. The MLST data were more relevant in terms of the overall population structure of the organisms. Both mutation and recombination appeared to be responsible for generating diversity among the isolates tested.

* Corresponding author. Mailing address: Bacteriology and Enteric Pathogens Program, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba R3E 3R2, Canada. Phone: (204) 789-2094 Fax: (204) 789-5012. E-mail: Clifford_Clark{at}phac-aspc.gc.ca.

Journal of Clinical Microbiology, May 2005, p. 2080-2091, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2080-2091.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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