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Journal of Clinical Microbiology, May 2005, p. 2215-2219, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2215-2219.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium

Mohamed K. Fakhr,1 Lisa K. Nolan,2 and Catherine M. Logue1*

Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota 58105,1 Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 500112

Received 24 August 2004/ Returned for modification 30 September 2004/ Accepted 17 January 2005

Nontyphoidal salmonellae are among the leading causes of food-borne disease in the United States. Because of the importance of Salmonella enterica in food-borne disease, numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the "gold standard" technique in typing Salmonella. The aim of this study was to compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing Salmonella enterica serovar Typhimurium clinical isolates. A total of 85 Salmonella Typhimurium clinical isolates from cattle were used in this study. PFGE using XbaI was performed on the 85 isolates by the Centers for Disease Control and Prevention method, and data were analyzed using the BioNumerics software package. Fifty PFGE profiles were observed among the isolates, and these grouped into three major clusters. For the MLST analysis, the manB, pduF, glnA, and spaM genes were amplified by PCR from the same 85 isolates. DNA sequencing of these four genes, manB, pduF, glnA, and spaM, showed no genetic diversity among the isolates tested, with a 100% identity in nucleotide sequence. Moreover, the DNA sequences of the aforementioned genes showed 100% identity to the sequence reported in GenBank for the S. enterica serovar Typhimurium LT2 strain. Therefore, MLST, using these genes, lacks the discriminatory power of PFGE for typing Salmonella enterica serovar Typhimurium.

* Corresponding author. Mailing address: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58105. Phone: (701) 231-7692. Fax: (701) 231-9692. E-mail: Catherine.logue{at}ndsu.nodak.edu.

Journal of Clinical Microbiology, May 2005, p. 2215-2219, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2215-2219.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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