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Journal of Clinical Microbiology, May 2005, p. 2224-2230, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2224-2230.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Phylogeny and Identification of Enterococci by atpA Gene Sequence Analysis

S. Naser,1,2* F. L. Thompson,2 B. Hoste,1,2 D. Gevers,3 K. Vandemeulebroecke,2 I. Cleenwerck,2 C. C. Thompson,1,2 M. Vancanneyt,2 and J. Swings1,2

Laboratory of Microbiology,1 BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium,2 Bioinformatics & Evolutionary Genomics, Ghent University/VIB, Technologiepark 927, Ghent 9052, Belgium3

Received 13 September 2004/ Returned for modification 23 November 2004/ Accepted 6 December 2004

The relatedness among 91 Enterococcus strains representing all validly described species was investigated by comparing a 1,102-bp fragment of atpA, the gene encoding the alpha subunit of ATP synthase. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. However, atpA gene sequences were much more discriminatory than 16S rRNA for species differentiation. All species were differentiated on the basis of atpA sequences with, at a maximum, 92% similarity. Six members of the Enterococcus faecium species group (E. faecium, E. hirae, E. durans, E. villorum, E. mundtii, and E. ratti) showed >99% 16S rRNA gene sequence similarity, but the highest value of atpA gene sequence similarity was only 89.9%. The intraspecies atpA sequence similarities for all species except E. faecium strains varied from 98.6 to 100%; the E. faecium strains had a lower atpA sequence similarity of 96.3%. Our data clearly show that atpA provides an alternative tool for the phylogenetic study and identification of enterococci.

* Corresponding author. Mailing address: Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium. Phone: 32-9-264 5238. Fax: 32-9-264 5092. E-mail: Sabri.Naser{at}Ugent.be.

Journal of Clinical Microbiology, May 2005, p. 2224-2230, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2224-2230.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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