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Journal of Clinical Microbiology, May 2005, p. 2241-2245, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2241-2245.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Investigation of a Nosocomial Outbreak of Imipenem-Resistant Acinetobacter baumannii Producing the OXA-23 ß-Lactamase in Korea

Byung-Chan Jeon,1 Seok Hoon Jeong,2 Il Kwon Bae,2 Su Bong Kwon,2 Kyungwon Lee,3 Dongeun Young,3 Jung Hun Lee,4 Jae Seok Song,4 and Sang Hee Lee4*

Department of Neurosurgery,1 Department of Laboratory Medicine, Kosin University College of Medicine, Busan 602-702,2 Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752,3 Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Kyunggido, 449-728, Republic of Korea4

Received 6 September 2004/ Returned for modification 23 November 2004/ Accepted 22 December 2004

We investigated an outbreak of Acinetobacter baumannii in an intensive care unit and in the surgery, medicine, neurology, and urology wards of the Kosin University Gospel Hospital in Busan, Korea. The outbreak involved 36 cases of infection by A. baumannii producing the OXA-23 ß-lactamase over an 8-month period and was caused by a single pulsed-field gel electrophoresis clone. The epidemic isolates were characterized by a modified cloverleaf synergy test. Isoelectric focusing of crude bacterial extracts detected one nitrocefin-positive band with a pI value of 6.65. PCR amplification and characterization of the amplicons by direct sequencing indicated that the epidemic isolates carried a blaOXA-23 determinant. The epidemic isolates were characterized by a multidrug resistance phenotype that remained unchanged over the outbreak, including penicillins, cephamycins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. This study shows that the blaOXA-23 resistance determinant may become an emerging therapeutic problem.

* Corresponding author. Mailing address: Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Kyunggido, 449-728, Republic of Korea. Phone: 82-31-330-6195. Fax: 82-31-335-8249. E-mail: sangheelee{at}mju.ac.kr.

Journal of Clinical Microbiology, May 2005, p. 2241-2245, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2241-2245.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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