This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Konno, M.
Right arrow Articles by Sugiyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Konno, M.
Right arrow Articles by Sugiyama, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2005, p. 2246-2250, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2246-2250.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Five-Year Follow-Up Study of Mother-to-Child Transmission of Helicobacter pylori Infection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method

Mutsuko Konno,1* Nobuhiro Fujii,2 Shin-ichi Yokota,2 Kiyoshi Sato,2 Michiko Takahashi,1 Kohei Sato,1 Emi Mino,1 and Toshiro Sugiyama3

Department of Pediatrics, Sapporo Kosei General Hospital,1 Department of Microbiology, Sapporo Medical University,2 Department of Gastroenterology, Hokkaido University Graduate School of Medicine, Sapporo, Japan3

Received 14 September 2004/ Returned for modification 18 October 2004/ Accepted 22 December 2004

Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serology and the stool antigen test. When infection of the children did occur, the strains from the children were compared to those of their mothers using DNA analysis. Sixty-nine of the 350 pregnant mothers (19.7%) had a positive serology for H. pylori. Fifty-one children underwent serological examinations and stool antigen tests at 4 to 6 days after birth, followed by 1, 3, and 6 months. They were continuously given the stool antigen test at 4- to 6-month intervals until the age of 5 years. Gastric juice samples were collected from the infected children and their mothers for culture and DNA analyses using a random amplified polymorphic DNA fingerprinting method. None of the 51 children acquired H. pylori infection during the first year of life. Of the 44 children enrolled in a 5-year follow-up study, five (11%) acquired H. pylori infection. They acquired the infection at the age of 1 year 2 months, 1 year 3 months, 1 year 6 months, 1 year 8 months, and 4 years 4 months. Random amplified polymorphic DNA fingerprinting confirmed that the strains of the five children exhibited DNA fingerprinting patterns identical to those of their mothers. These findings suggest that mother-to-child transmission is the most probable cause of intrafamilial spread of H. pylori.

* Corresponding author. Mailing address: Department of Pediatrics, Sapporo Kosei General Hospital, N-3, E-8, chuo-ku, Sapporo 060-0033, Japan. Phone: 81-11-261-5331. Fax: 81-11-271-5320. E-mail: mutsukon{at}ja-hokkaidoukouseiren.or.jp.

Journal of Clinical Microbiology, May 2005, p. 2246-2250, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2246-2250.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oshio, I., Osaki, T., Hanawa, T., Yonezawa, H., Zaman, C., Kurata, S., Kamiya, S. (2009). Vertical Helicobacter pylori transmission from Mongolian gerbil mothers to pups. J Med Microbiol 58: 656-662 [Abstract] [Full Text]  
  • Herrera, P. M., Mendez, M., Velapatio, B., Santivaez, L., Balqui, J., Finger, S. A., Sherman, J., Zimic, M., Cabrera, L., Watanabe, J., Rodriguez, C., Gilman, R. H., Berg, D. E. (2008). DNA-Level Diversity and Relatedness of Helicobacter pylori Strains in Shantytown Families in Peru and Transmission in a Developing-Country Setting. J. Clin. Microbiol. 46: 3912-3918 [Abstract] [Full Text]  
  • Jodlowski, T. Z, Lam, S., Ashby, C. R Jr (2008). Emerging Therapies for the Treatment of Helicobacter pylori Infections. The Annals of Pharmacotherapy 42: 1621-1639 [Abstract] [Full Text]  
  • Matteo, M. J., Granados, G., Perez, C. V., Olmos, M., Sanchez, C., Catalano, M. (2007). Helicobacter pylori cag pathogenicity island genotype diversity within the gastric niche of a single host. J Med Microbiol 56: 664-669 [Abstract] [Full Text]  
  • Solnick, J. V., Fong, J., Hansen, L. M., Chang, K., Canfield, D. R., Parsonnet, J. (2006). Acquisition of Helicobacter pylori Infection in Rhesus Macaques Is Most Consistent with Oral-Oral Transmission.. J. Clin. Microbiol. 44: 3799-3803 [Abstract] [Full Text]  
  • Kusters, J. G., van Vliet, A. H. M., Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev. 19: 449-490 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»