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Journal of Clinical Microbiology, May 2005, p. 2266-2273, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2266-2273.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)

Monica C. Galiano,1 Concepcion Palomo,2 Cristina M. Videla,1* Juan Arbiza,3 José A. Melero,2 and Guadalupe Carballal1

Laboratorio de Virología Clínica, Centro de Educacion Médica e Investigaciones Clínicas, CEMIC, Hospital Universitario, Av. Galván 4102, Buenos Aires (C1431FWO), Argentina,1 Unidad de Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220, Madrid, Spain,2 Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay3

Received 5 August 2004/ Returned for modification 1 October 2004/ Accepted 10 January 2005

The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed.

* Corresponding author. Mailing address: Laboratorio de Virología Clínica, CEMIC Galván 4102, Buenos Aires (C1431FWO), Argentina. Phone: (54) 11 4546 8228. Fax: (54) 11 4541 3790. E-mail: cvidela{at}cemic.edu.ar.

Journal of Clinical Microbiology, May 2005, p. 2266-2273, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2266-2273.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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